EST 7987

Library
Name: 0089P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0007
Well: E05
Primer:
Genbank record: CV518039
Genbank sequence name: 0089P0007Z_E05_T7
Trace file: 568521249
Unigene: MgU1732 MgU9589 MgU92835
sequence: CATTGGGCCC GACGTCGCAT GCTCCCGGCC GCCTGGCGGC CGCGGGAATT CGATTAAGCA GTGGTATCAA CGCAGAGTAC TTTTTTTTTT TTTTTTTTTT TTTTTTTTTG GGCATGACAT ACTTCTTGAA CAATAAACTA ATATTGTCCA ACGACGATAT AAAAAAAACA CTCGCTTATA TATGGGCGTA TAAAACTCGC AGTGGACATC TTTCTTATTA CATCGATCAC TGAACCAAAC GACACAAACC GAATTATTGT AATTATTAAT CAGGCCTCCA GCAAGAACCA GGCTCTACAA ATGTATGGCT AAAGACGAGA AAAATTCAAC GCTTTTATTT CTGCTGACTA TACCGTAAAA TCCCATAAAC ATCGACAATG CCAGGTGGAA AAACGGGATC GCTTGCCGCT CTTACAGCAA CTCTAGCAAC AGCATTAACA TTAACAGGAG GGGTTAATAG TGGCCCCACT AGCGGGATCT GGCTCAGTGG CTTAACATGC TGCAGCACCC TCTCCAAAGG AGAGCCAATC ACGCCAAGTG GTAATTTCAT GCTCCCAACA CGACGAGTTC CATATATGAA ACCAGGCCTC AGAATCACTC CTCCATACGC ATACTTAGTG AGTAACTCTG TCTCAGCGGC TCTCTTCCCC TCATAGTATC CTTGTAGCAG GTAATTTACC ACCCCAAAGT CAGCAGCAGA GATGTACACA AACCTCTTAA CTCCTTCATC TGAAGCAGCC CTAATAGCGT TAATATTTGC GGTCCC