EST 6737

Library
Name: 0089P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0002
Well: D08
Primer:
Genbank record: CV517425
Genbank sequence name: 0089P0002Z_D08_SP6
Trace file: 568520635
Unigene: MgU1221 MgU10629 MgU94088
sequence: GTACTCAGCT AGCATCCACG CGTTGGGAGC TCTCCCTATG GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTAAGC AGTGGTATCA ACGCAGAGTA CTTTTTTTTT TTTTTTTTTT TTTTTTTGGA TAGATCAATC CATAAACAAA ATCGCAATAA CTTAAGGTGT CCGTACACAA CATACGTCGG GATCATGCAG CAAGTTCTAT TGGGGGTGTA ATAATCAACG AAAACAGAAA CAGGTTTACG AAAGCAAAAA CATAACAAAT TCCATCACAT TTAAAAGTCC TAGAAAAGAA ATCGAAATCA AAAAATCCAG TCCTTCTTTG GCAGCTCTCT TAACGGGTAG CCTTGGCAAC CCTCTCGATC TCATCCTTCT TCCCGCGTAC TCTGCGTTGA TACCACTGCT TAATCGAATT CCCGCGGCCG CCATGGCGGC CGGGAGCATG CGACGTCGGG CCCAATTCGC CCTAAAGTGA GTCGTATTAC AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CAAAAGTTGC CCACCCTGAA GGCGAAAGGA CCCGCCCTGT ACGGCCCATT ACCCCGGCGG GGGGGGTGGT TCCCCCAACG GGGCCGGTAC ACTTGCAGCG CCCTAACGCC CGCTCCTTTC CGTTTCTTTC CT