EST 6309

Library
Name: 0048P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0011
Well: E07
Primer:
Genbank record: CV515864
Genbank sequence name: 0048P0011Z_E07_SP6
Trace file: 568475483
Unigene: MgU836 MgU9502
sequence: ATGATACGCC AGCTCGAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG CTCTAGAACT AGTGGATCCC CCGGGCTGCA GGAATTCGGC ACGAGGGTCT CGTTTTTTTT TTTTTTTTTT TATACAAAGA GTAATTTATA TTGTGCTGCA TAGCATAAAA ATAATAACCG AGTGGCCATT GTGGCTTACA AAGGGCGCCG AGAGGGAGGT TGCAAATAAG TTCAATTTTG TTATCAAATG GTGGGATTCA AAATCCTGCC ATTTATTAGG CACAAATGTT TCGGTCGATT CTTACATAAT AATCACATGA AAAATTGTCG CATCACATAT GTTCTTTTGT TCAATTTCTT TGGTGCGGTT TCTCTGCTAA AAGACGCTTT ACATTTCTCC TGGAAGCATT GTATCTCCCG GAAGATCACA CACAGCGATA CCCTCTCCAG TGAGATGGGC TACACATTTC TTCTCAGCAA GTAGCTCTGC TTCTGTCTTC TTGATTCGAT TCCAAAGGTA GTCGTCAAAC ACTACATCCT TGACAAGTTC ATAATCACCA TCTCCTTTAG ATCTGCACGG CATACTGAAG ACAATGTCAT CTGATATACC GTATGGATTT CCAGCCGTAT ATACTCCGGT TGAAAACCAA TCTCCGTCGG GTGTCGGATT CACGAGTGAC CTGATTGCAT CAACAATCGA GACAGCAGTC GATGCGGCAG AGGATCGTCC CCATTTCTTA ATAAGGACGC CACCTCTCGT CTGAACTTTC TCCGTGAACT CCTCT