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Library

Name:

0048P

Strain:

IM62

Tissue:

tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems

Host:

DH5alpha

Vector:

Plasmid pGEM-T Easy

Description:

IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.

EST

Plate:

0018

Well:

A10

Primer:

Genbank record:

CV516961

Genbank sequence name:

0048P0018Z_A10_T7

Trace file:

568476580

Unigene:

MgU374

sequence:

NGATTGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC GAGTTTTTTT TTTTTTTTTT TAATGAAACA CACCACGCTA AATAAACACA ACAATTCAGA ATTAGTAGGG ATGCATTAAC AGAAGAGATA ACTAGCAACA GCAACAAAAA AAAATGCCTC AATAGGAACA ATAGGTAAAG GGGAAAGCAT TAGAGTATTT GTACAAATAG GCCAACTATT TTCTTGACAA TTGCCCCCCT TGTGTATTCT CTAACAACGA CTCTAGTGAC CGTACTTCTG AAACTCCCAC TCGCTGTTAT CAAGAGGGCA AACTTGACGA GTCTTAAGCC ACCTGCTAAT ACAGTGGAAG TGGAACGCAT GGTTGCAAAC TCCCCAAGCA ACAGTGCATT CCTCGCTGGT GGCACTAGCT TGATTAGCCT GACACTCGAT GCAGAGATCC ATGATGTGGT TCCTGCAAAT CGCACAATTA TCCACCACGA TATCCCAAGC CCAGAGCGAG ACGGCGTTCC ACTTTTTGAT CTCGAACACC TTCCCCTTCT TGGCGGACTG CGGCGGCGGC AGCGTCGTGA GATCGGTGGC GGTGGAAGGT CCGGCACCGG TGGATCCCTC TGCGACGGGA ACCATACTTG CGTCGGTTTC ATGTGAAGTC ATCTTCGCTT TTGGCTTTGG TTNTAGCTTT ACCCCTCGTG CCGAATTCCT GCAGCCCGGG GGATCCACTA GTTCTAGAGC GGCCGCCACC GCGGTGGAGC TCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTTCGAGCT TG

EST 5122