EST 5003

Library
Name: 0048P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0015
Well: C10
Primer:
Genbank record: CV516549
Genbank sequence name: 0048P0015Z_C10_SP6
Trace file: 568476168
Unigene: MgU314 MgU9934
sequence: ATGATACTCC AGCTCGAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG CTCTAGAACT AGTGGATCCC CCGGGCTGCA GGCATTTTTC CATGGCGGTA GTGCTCGGCA ACGTTTCTCC GTTCCTCGAC CTCTCCACCT CGCCGCCTCG GCCCATAATC CCCGACCGCC GCCTCCGCCC CTTCCCCTCC GACGCGCTCC TGAACCTCTT CAACAAAGAC CTCCACCAGA ACCCTAATTT CCACGCGGCG TTCGAATCGG TGTTCGATAA CCGGGAAAGG CACTCGAACA AGCGGAAATC GAACCAGTCG AAGGTGAACG GAGTGGAGTA CGACAATTCC AGCGACGACC AGAACGGCGG CAGCGGGAGC GGGTTCGAGG ATGAGCTCGA AGATGAAGCC GATGAATTCA ACTGGGAGAG GGAGATGAGG AAGAGAGTGA AGGAAATTGA AGAAATGAGG GAGTTGGAGA AGAAAGCCGA AGAATTGCAG TACAAAGTCG ATCAGGAGTT CGGCGCCGGC GGCGGTGGCA ACGGCCAGCC GGATGACGAA ACTGAAGAAC AGAAGCGCAT GAGGGTTAAG AAAGAACTAG AAAAGGTAGC ATAGGAGCAA GCAGAACGAA GGAGAACCGC TCAATTGATG TTCGATTTGG GGCAGAAAGC ATACGGAAGG GGTATGTATG GACGCGCCAT TGAATTTCTC GAAGGTGCAC TCACAATCAT TCCCAGGCCA ACCTTGTTCG GGCGTGAGAT ACAAATATGG CTGGN