EST 4677

Library
Name: 0048P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0009
Well: H02
Primer:
Genbank record: CV515537
Genbank sequence name: 0048P0009Z_H02_SP6
Trace file: 568475156
Unigene: MgU151 MgU9628 MgU93648
sequence: ATGATTACGC CAAGGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCTCTAG AACTAGTGGA TCCCCCGGGC TGCAGGCTTC CGTACACCCT CGTTCCTTCC CCACCGTCGA TTTCACCTCC TCCTTCAACG TACAATCACT CAAAGCACAA CTCTCTCCCC CAATTCAACC CCCCGTACTA CACTTACTCC ATTTGCTTCA ATTTCGTCAA TGTCTTCCTC TTTCTACCCG GAAAAAGCTC GGGTTCCTCC AGCGATCCAA TCGCCTACTC CTCCCATCAC TAAGTTTAAA ATTGGATTAT GCCAATTGAG AGTAACTGCC GACAAGCTAA GGAACATTTT ACATACTCGT ACTGCATTAG AGGAAGCTGC CGCGAAGGGC GCAAAGCTTG TTGTGTTGCC CGAAATATGG AATAGTCCAT ACTCGAATGA TAGTTTTCCA GTGTATGCTG AGGATATTGA TTCCGGTTTC GATGCATCTC CATCGACAGC AATGTTGTCT GAAGTTGCGC GTCTTCTTAA AATTACGATA ATCGGAGGTT CGATTCCGGA AAAAAGTGGC GGGAAATTGT ATAACACGTG CTGTGTATTC GGACCCGATG GAAACCTGAA AGCTAAACAT CGTAAGATAC ATCTTTTTGA TATCGATATT CCTGGGAAGA TTACGTTCAA GGAATCGAAG ACTCTTACAG CTGGAGAAAC TCCCACTACA GTAGACACAG AGGTTGGTCG AATTGGAATA GGTATCTGCT ACGACATTCG