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Library

Name:

0048P

Strain:

IM62

Tissue:

tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems

Host:

DH5alpha

Vector:

Plasmid pGEM-T Easy

Description:

IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.

EST

Plate:

0008

Well:

E11

Primer:

Genbank record:

CV515333

Genbank sequence name:

0048P0008Z_E11_SP6

Trace file:

568474952

Unigene:

MgU16 MgU93439

sequence:

ATGATACTCC AGCTCCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG CTCTAGAACT AGTGGATCCC CCGGGCTGCA GGAATTCGGC ACGAGGCTCC GATTAATCGG CATTGTTTTG TAATGGATTC GTGTTGCTAT TGTGTTTTTC AAGGAGTGTT TTCTGAATCG ACAATGGCGG ATCAGCACCC AACCGTTGCT CAGAAGGTAG CCAATCAGCT GCATCTCAGC TCCACTTTCT CTCACAACAT CCAATCCGGC TATGGTGGCT TCCAGAAGCC TTCTGTGTAC CAAAGGCATT TCTCGTACGG CAATTACACA AACGGAGGAT TGAAGTACCC CACATCTTGC CGAGCCAGTC AGGATCTTTC GCTGATCACC GCAAATGCTT CGCCGGTGTT TGTCCAGGCT CCAGCGGAGA AAGGCTTTGC CAGTTTCGCC ATCGATTTTC TCATGGGCGG AGTTTCGGCC GCCGTGTCGA AAACTGCCGC TGCACCTATC GAGCGTGTGA AGCTTCTGAT CCAGAACCAA GACGAAATGC TTAAAGCTGG CAGGCTCTCG GAACCCTACA AGGGAATCGG CGAATGTTTC AAGAGAACAA TTTCGGGAAA GAAGGGTTTG GATCTCTCTG GAGAGGACAC ACCGGTTACG TCATTTCGTT ACTTTCCCAC TCAGGCTTTG AACTTTGCAT TCAAGGACTA TTTCAAGAGA CTTTTCACTT TCAAGAAAGA CCGCGATGGC TTACTGGAAT GGTTCGCCGG CAACCTTGCG TCTGGTGGT

EST 4459