EST 1522764
| Library | |
| Name: | |
| Strain: | IM62 |
| Tissue: |
tips of developing inflorescences, consisting of very young unopened floral buds (< 5mm long), floral meristems, and shoot apical meristems |
| Host: | DH5alpha |
| Vector: | Plasmid pGEM-T Easy |
| Description: |
IM62 is a highly inbred line propagated for > 10 generations by self-fertilization and derived from a predominantly outcrossing (~80%), annual population of M. guttatus located on Iron Mountain, Linn Co., Oregon. Plants were grown from seed in the greenhouses at Duke University and tissue for RNA extraction was collected from 1-2 month old plants. RNA was extracted using the standard protocol for the Qbiogene FastRNA Pro Green kit. cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit. No size selection was performed. Library was not normalized and was not directional. Titer of primary library was ~1000 cfu/ng vector (pGEM-T Easy). High quality start and High quality end are determined by phred score >= 20 for the final base, and an average phred score >= 20 for the final 10 bases. Sequence ends were determined using cross_match to remove vector, and with ends of average phred score <= 11 removed, and homopolymeric end sequences of phred score <= 15 removed. Contaminants (bacterial and fungal) were screened for and excluded. Project support provided by NSF FIBR. |
| EST | |
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| Trace file: | |
| Unigene: | MgU3859 |
| sequence: | GAGAGAGAGA GAGAGAGGCA TATAGCACAG TGTACAAAGA AGATCAGCGG AAAGTAGCGT AGTAGAGAGA GAGAGAGAGG GGTGAAAATG AGCAGCATAG GCACAGGCTA CGATCTGTCA GTAACGACAT TCTCGCCGGA CGGCCGCGTT TTCCAGATCG AGTACGCCGC CAAAGCCGTC GACAACAGCG GTACGGTCGT CGCAATCAAA TGCAAAGACG GTGTCGTAAT GGGAGTGGAG AAGCTGATTG CTTCAAAAAT GATGTTGCCC GGGTCCAATA GAAGAATCCA CGCCGCCCAT CGCCACTCCG GCATGGCTGT TGCTGGATTA GCTGCAGATG GCAGGCAGAT TGTGACAAGA GCAAAATCTG AAGCAACTAA CTATGAGAGT GTGTATGGAG AACCAATTCC TGTAAAGGAA CTTGCAGAAC GTGTCGCCAG TTATGTGCAT CTGTGCACAC TATATTGGTG GCTAAGGCCT TTTGGTTGTG GTGTAATTCT GGGAGGTTAT GATAGGGATG GGCCACAGTT ATACATGATT GAACCATCTG GCATATCCTA TCGATACTTT GGAGCATCCA CTGGAAAAGG AAGGCAGGCT GCGAAAACGG AGATCGAAAA TTAAAGCTTT CAGATATGAC ATGTCGACAA GGTGTTATTG AGGCGGCCAA AATTATTTAT GGGGTGCATG ATGAGGCCAA GGACAAAGCC TTTGAATTGA AATGAGTGGG TCTGTGATGA GTCGAATCGC ACATCAAAAG TT |