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Library

Name:

0089P

Strain:

IM62

Tissue:

tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems

Host:

DH5alpha

Vector:

Plasmid pGEM-T Easy

Description:

IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.

EST

Plate:

0096

Well:

G03

Primer:

Genbank record:

DV207488

Genbank sequence name:

0089P0096Z_G03_T7

Trace file:

1066108734

Unigene:

MgU768 MgU93457

sequence:

CATTGGGCCC GACGTCGCAT GCTCCCGGCC GCCTGGCGGC CGCGGGAATT CGATTAAGCA GTGGTATCAA CGCAGAGTAC TTTTTTTTTT TTTTTTTTTT TTTTTTTTTA AAATAATAAA AACGACGGAT AGAAGAATAT AGCAATGGTC CAGTCTCGAT AGGATTGAAA AATGCATGCT CCGACAAATT TAAACTAACA TCTAGTCATG CAAAGAAAAA AAATAATATT ACTATTAAGC AGTTGCCGAT TGGGCAGCGA GCCTCTTCCT GGCCTCCTCA ATGATAGTAA ACTTAATACC TTTGCCCTTT GGAAGAAAAA CCCACGGCTT TGTACCCTTT CCATGGTGTA CACGTTGCTC AATCGTGTAG CAAATTCATT GCCAGTTGAA TCCTGAATGT GAACAGTCTC AAAGCTACCC TTATGCTTCT CACGATTCTT GAGTATTCCA ACACGGCCTC TGTTCCTACT ACCAGTCACC ATCACAACAT TTCCGACATC GAATTTGATA AAGTCGACAA TCTTGTTGCT GTCCAAATCC AGCTTGATGG TGTCGTTTGC CTTGATAAGT GGGTCCGGGT AGCGAATGGT CCTTCCATCG TATGTGTTGA TGTACGGAAT ACCCTTCTTT CAGAACTGCC CGCGTACTCT GCGTTGATAC CACTGCTTAA TCACTAGTGA ATTCCCGGCC GCCTGCCGGT CGACCATATG GGAAAGCTCC CACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGCCAC CAAATAGGTT GGCGTAACAT GGGTAT

EST 14243