EST 13655

Library
Name: 0089P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0084
Well: C06
Primer:
Genbank record: DV206683
Genbank sequence name: 0089P0084Z_C06_T7
Trace file: 1066107929
Unigene: MgU1221 MgU10632 MgU94088
sequence: CAATTGGGCC CGACGTCGCA TGCTCCCGGC CGCCAGGCGG CCGCGGGAAT TCGATTAAGC AGTGGTATCA ACGCAGAGTA CGCGGGCAGG AATGTTAAGA GCATTGCTGA ATGCCTTGCT GATGAACTCA TCAATGCTGC TAAAGGATCG TCGAACAGCT ACGCGATCAA GAAGAAGGAT GAGATCGAGA GGGTTGCCAA GGCTAACCGT TAAGAGAGCT GCCAAAGAAG GACTGGATTT TTTGATTTCG ATTTCTTTTC TAGGACTTTT AAATGTGATG GAATTTGTTA TGTTTTTGCT TTCGTAAACC TGTTTCTGTT TTCGTTGATT ATTACACCCC CAATAGAACT TGCTGCATGA TCCCGACGTA TGTTGTGTAC GGACACCTTA AGTTATTGCG ATTTTGTTTA TGGATTGATC TAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA ACCAAAAAAA AAAAAAAAAA AAAAATTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAGGGT ACTCGGCGTT GATCCCACTG CTTAACCACT AGGGAATTCG CGGCCGCCTG CGGGTCGACC ATATGGGAGA GCTCCCAACG CGTTGGATGC ATAGCTTGAG TATTCTATAG GGCCACCTAA AAAGCTGGCC GTAACCAGGG CCAAAGCTGT TTCCGGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCG GGAAGCATAA AGTGTAAACC CGGGGGGGCC AAGGGAGGGA GCTAACTCAC ATTTATTGGG TTGGGCTTAC GTGCCGTTTT CA