EST 13654

Library
Name: 0089P
Strain: IM62
Tissue: tips of developing inflorescences, consisting of very young
unopened floral buds (< 5mm long), floral meristems,
and shoot apical meristems
Host: DH5alpha
Vector: Plasmid pGEM-T Easy
Description: IM62 is a highly inbred line propagated for > 10 generations by
self-fertilization and derived from a predominantly outcrossing
(~80%), annual population of M. guttatus located on Iron Mountain,
Linn Co., Oregon. Plants were grown from seed in the greenhouses
at Duke University and tissue for RNA extraction was collected
from 1-2 month old plants. RNA was extracted using the standard
protocol for the Qbiogene FastRNA Pro Green kit. cDNA was
synthesized from total RNA using the BD Biosciences SMART PCR
cDNA synthesis kit. No size selection was performed. Library
was not normalized and was not directional. Titer of primary
library was ~1000 cfu/ng vector (pGEM-T Easy). High quality
start and High quality end are determined by phred score >= 20
for the final base, and an average phred score >= 20 for the final
10 bases. Sequence ends were determined using cross_match to
remove vector, and with ends of average phred score <= 11
removed, and homopolymeric end sequences of phred score <= 15
removed. Contaminants (bacterial and fungal) were screened for and
excluded. Project support provided by NSF FIBR.
EST
Plate: 0073
Well: F12
Primer:
Genbank record: DV206016
Genbank sequence name: 0089P0073Z_F12_T7
Trace file: 1066107262
Unigene: MgU1221 MgU10632 MgU94088
sequence: CATTGGGCCC GACGTCGCAT GCTCCCGGCC GCCTGGCGGC CGCGGGAATT CGATTAAGCA GTGGTATCAA CGCAGAGTAC GCGGGAGGTC ATTGTTGATG CTGTCATCAA CAGTGGTCCG AGGGAAGATG CAACCAGGAT TGGTTCAGCT GGTGTTGTGA GGCGTCAAGC TGTTGATATC TCACCTCTTC GCCGTGTGAA TCAGGCCATT TATCTCATCA CAACTGGTGC TAGGGAATCC TCTTTCAGGA ATGTTAAGAG CATTGCTGAA TGCCTTGCTG ATGAACTCAT CAATGCTGCT AAAGGATCGT CGAACAGCTA CGCGATCAAG AAGAAGGATG AGATCGAGAG GGTTGCCAAG GCTAACCATT AAGAGAGCTG CCAAAGAAGG ACTGGATTTT TTGATTTCGA TTTCTTTTCT AGGACTTTTA AGTGTGATGG AATTCGTTAT GTTTTTGCTT TCGTAAACCT GTTTCTGTTT TCGTTGATTA TTACACCCCC AATAGAACTT GCTGCATGAT CCCGACGTAT GTTGTGTACG GACACCTTAA GTTATTGCGA TTTTGTTTAT GGATTGATCT ATATAGCTAT TTGTTTTGCN NANANAAAAN NAAAAAAAAA AAAAAGTACT CTGCGTTGAT ACCACTGCTT AATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACCATA TGGGAGAGCT CCCAACGCGT TGGATGCATA GCTTGAGTAT TCTATAGTGT CACCTAAATA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTG