EST 1081378
| Library | |
| Name: | |
| Strain: | IM62 |
| Tissue: |
tips of developing inflorescences, consisting of very young unopened floral buds (< 5mm long), floral meristems, and shoot apical meristems |
| Host: | DH5alpha |
| Vector: | Plasmid pGEM-T Easy |
| Description: |
IM62 is a highly inbred line propagated for > 10 generations by self-fertilization and derived from a predominantly outcrossing (~80%), annual population of M. guttatus located on Iron Mountain, Linn Co., Oregon. Plants were grown from seed in the greenhouses at Duke University and tissue for RNA extraction was collected from 1-2 month old plants. RNA was extracted using the standard protocol for the Qbiogene FastRNA Pro Green kit. cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit. No size selection was performed. Library was not normalized and was not directional. Titer of primary library was ~1000 cfu/ng vector (pGEM-T Easy). High quality start and High quality end are determined by phred score >= 20 for the final base, and an average phred score >= 20 for the final 10 bases. Sequence ends were determined using cross_match to remove vector, and with ends of average phred score <= 11 removed, and homopolymeric end sequences of phred score <= 15 removed. Contaminants (bacterial and fungal) were screened for and excluded. Project support provided by NSF FIBR. |
| EST | |
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| Trace file: | |
| Unigene: | MgU374 |
| sequence: | GCGAAGATGA CTTCACATGA AACCGACGCA AGTATGGTTC CCGTCGCAGA GGGATCCACC GGTGCCGGAC CTTCCACCGC CACCGATCTC ACGACGCTGC CGCCGCCGCA GTCCGCCAAG AAGGGGAAGG TGTTCGAGAT CAAAAAGTGG AACGCCGTCT CGCTCTGGGC TTGGGATATC GTGGTGGATA ATTGTGCGAT TTGCAGGAAC CACATCATGG ATCTCTGCAT CGAGTGTCAG GCTAATCAAG CTAGTGCCAC CAGCGAGGAA TGCACTGTTG CTTGGGGAGT TTGCAACCAT GCGTTCCACT TCCACTGTAT TAGCAGGTGG CTTAAGACTC GTCAAGTTTG CCCTCTTGAT AACAGCGAGT GGGAGTTTCA GAAGTACGGT CACTAGAGTC GTTGTTAGAG AATACACAAG GGGGGCAATT GTCAAGAAAA TAGTTGGCCT ATTTGTACAA ATACTCTAAT GCTTTCCCCT TTACCTATTG TTCCTATTGA GGCATTTTTT TTTGTTGCTG TTGCTAGTTA TCTCTTCTGT TAATGCATCC CTACTAATTC TGAATTGTTG TGTTTATTTA GCGTGGTGTG TTTCATTATT TGATTTATCG CGTATGGTAT TCAATCTTAA TTCGAAGCAG AACGGGCTTG GAAATTGCTG CATACTTGAT TAAAATCCTT TGAACAAAGC AAGAGTTTGA G |