EST 1063685
| Library | |
| Name: | |
| Strain: | IM62 |
| Tissue: |
tips of developing inflorescences, consisting of very young unopened floral buds (< 5mm long), floral meristems, and shoot apical meristems |
| Host: | DH5alpha |
| Vector: | Plasmid pGEM-T Easy |
| Description: |
IM62 is a highly inbred line propagated for > 10 generations by self-fertilization and derived from a predominantly outcrossing (~80%), annual population of M. guttatus located on Iron Mountain, Linn Co., Oregon. Plants were grown from seed in the greenhouses at Duke University and tissue for RNA extraction was collected from 1-2 month old plants. RNA was extracted using the standard protocol for the Qbiogene FastRNA Pro Green kit. cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit. No size selection was performed. Library was not normalized and was not directional. Titer of primary library was ~1000 cfu/ng vector (pGEM-T Easy). High quality start and High quality end are determined by phred score >= 20 for the final base, and an average phred score >= 20 for the final 10 bases. Sequence ends were determined using cross_match to remove vector, and with ends of average phred score <= 11 removed, and homopolymeric end sequences of phred score <= 15 removed. Contaminants (bacterial and fungal) were screened for and excluded. Project support provided by NSF FIBR. |
| EST | |
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| Trace file: | |
| Unigene: | MgU3859 |
| sequence: | ACCCTTTTTT TTTTTTTTTG GGTTTAATAA AATCCGATTG ACTTAACATC ATCTTTGGTG CCGTTTCGCG TATTTCCACA CCATGACCAA CATAATTCGA AGCAGAAAAA AATAAAATGA TCAAAGCAAA TGAGTGAAAA CTAATCTGCA TCCATTTCTT CAAGTGCAGC TTTAGCTGCT GCCTTAGCTT CCTCTAATAG ATTGTCTGGA ACCTTTTGAT GTTGGCGATT CGACTCATCA CAGACCCAAC TCATTTCCAA TTCAAAGGCT TTGTCCTTGG CCTCATCATG CACCCCATAA ATAATTTTGG CCACCTCAAT AACACCTTGT CGACATGTCA TATCTGAAAG CTTTAATTTT TCGATCTCCG TTTTCGCAGC CTGCCTTCCT TTTCCAATGG ATGCTCCAAA GTATCGATAG GATATGCCAG ATGGTTCAAT CATGTATAAC TGTGGCCCAT CCCTATCATA ACCTCCCAGA ATTACACCAC AACCAAAAGG CCTTAGCCAC CAATATAGTG TGCACAGATG CACATAACTG GCGACACGTT CTGCAAGTTC CTTTACAGGA ATTGGTTCTC CATACACACT CTCATAGTTA GTTGCTTCAG ATTTTGCTCT TGTCACAATC TGCCTGCCAT CTGCAGCTAA TCCAGCAACA GCCATGCCGG AGTGGCGATG GGCGGCGTGG ATTCTTCTAT TGGACCCGGG CAACATCATT TTTGAAGCAA TCAACTTCTC CACTCCCATT ACGACACCGT CTTTGCATTT GATTGCGACG ACGTACCGCT GTTGTCGACG GCTTTGGCGG GCGTACTCGA TCTGGAAA |