EST 1053696
| Library | |
| Name: | |
| Strain: | IM62 |
| Tissue: |
tips of developing inflorescences, consisting of very young unopened floral buds (< 5mm long), floral meristems, and shoot apical meristems |
| Host: | DH5alpha |
| Vector: | Plasmid pGEM-T Easy |
| Description: |
IM62 is a highly inbred line propagated for > 10 generations by self-fertilization and derived from a predominantly outcrossing (~80%), annual population of M. guttatus located on Iron Mountain, Linn Co., Oregon. Plants were grown from seed in the greenhouses at Duke University and tissue for RNA extraction was collected from 1-2 month old plants. RNA was extracted using the standard protocol for the Qbiogene FastRNA Pro Green kit. cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit. No size selection was performed. Library was not normalized and was not directional. Titer of primary library was ~1000 cfu/ng vector (pGEM-T Easy). High quality start and High quality end are determined by phred score >= 20 for the final base, and an average phred score >= 20 for the final 10 bases. Sequence ends were determined using cross_match to remove vector, and with ends of average phred score <= 11 removed, and homopolymeric end sequences of phred score <= 15 removed. Contaminants (bacterial and fungal) were screened for and excluded. Project support provided by NSF FIBR. |
| EST | |
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| Trace file: | |
| Unigene: | MgU3859 |
| sequence: | GATCAAACTT CACGGTGATG TGCATATCAT CGCAAGTGTA GAGTATACAT GATCATAGGT TTAATAAAAT CCGATTGACT TAACATCATC TTTGGTGCCG TTTCGCGTAT TTCCACACCA TGACCAACAT AATTCGAAGC AGAAAAAAAT AAAATGATCA AAGCAAATGA GTGAAAACTA ATCTGCATCC ATTTCTTCAA GTGCAGCTTT AGCTGCTGCC TTAGCTTCCT CTAATAGATT GTCTGGAACC TTTTGATGTT GGCGATTCGA CTCATCACAG ACCCAACTCA TTTCCAATTC AAAGGCTTTG TCCTTGGCCT CATCATGCAC CCCATAAATA ATTTTGGCCA CCTCAATAAC ACCTTGTCGA CATGTCATAT CTGAAAGCTT TAATTTTTCG ATCTCCGTTT TCGCAGCCTG CCTTCCTTTT CCAATGGATG CTCCAAAGTA TCGATAGGAT ATGCCAGATG GTTCAATCAT GTATAACTGT GGCCCATCCC TATCATAACC TCCCAGAATT ACACCACAAC CAAAAGGCCT TAGCCACCAA TATAGTGTGC ACAGATGCAC ATAACTGGCG ACACGTTCTG CAAGTTCCTT TACAGGAATT GGTTCTCCAT ACACACTCTC ATAGTTAGTT GCTTCAGATT TTGCTCTTGT CACAATCTGC CTGCCATCTG CAGCTAATCC AGCAACAGCC ATGCCGGAGT GGCGATGGGC GGCGTGATTC TTCTATTGGA CCCGGGCAAC ATCATTTTTG AGCATCACTT CTCCACTCCC ATTACGACAC CGTCTTTGCA TT |