EST 1048898
| Library | |
| Name: | |
| Strain: | IM62 |
| Tissue: |
tips of developing inflorescences, consisting of very young unopened floral buds (< 5mm long), floral meristems, and shoot apical meristems |
| Host: | DH5alpha |
| Vector: | Plasmid pGEM-T Easy |
| Description: |
IM62 is a highly inbred line propagated for > 10 generations by self-fertilization and derived from a predominantly outcrossing (~80%), annual population of M. guttatus located on Iron Mountain, Linn Co., Oregon. Plants were grown from seed in the greenhouses at Duke University and tissue for RNA extraction was collected from 1-2 month old plants. RNA was extracted using the standard protocol for the Qbiogene FastRNA Pro Green kit. cDNA was synthesized from total RNA using the BD Biosciences SMART PCR cDNA synthesis kit. No size selection was performed. Library was not normalized and was not directional. Titer of primary library was ~1000 cfu/ng vector (pGEM-T Easy). High quality start and High quality end are determined by phred score >= 20 for the final base, and an average phred score >= 20 for the final 10 bases. Sequence ends were determined using cross_match to remove vector, and with ends of average phred score <= 11 removed, and homopolymeric end sequences of phred score <= 15 removed. Contaminants (bacterial and fungal) were screened for and excluded. Project support provided by NSF FIBR. |
| EST | |
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| Trace file: | |
| Unigene: | MgU890 |
| sequence: | AGAAAATATT TACACCTGGC AAAAGTGGCA AAAGTACTGT TAATTTTATT TTCTGGAGTC TAACTCGTAC AATAAAATAA CTCGTGTGAT TTTGAAGATC AATTTACACC GTATAGTTGC TAGAGTAAAG GGAACAACCT AAATACAAAA CGATGTTATA AAAAATAACG AAGATATAAT TTTAACTTCT GCTATCAAGT GTAAGAGTGT AGAGTAGTAG AAAACAGCTC GTTTCAATCT CGAGTATGCA GCAGATTTAG ATCATCCGTT AAGAAGGGAA GTGTCCTGTT CAATTTCTTT CCGGGAAGCT TCCCAAAGCC GTTGCTTGAT TCCCAATTTC CTAATCTCCA TTAGACCTTG CTCAACATCG ACTGCGTCAT GCGAAGTTGG TGACTGGCCG CTGCGGCCGA TCCAGAATTT CAGTCTCTCG TTTGCAATTT CCTCTTCTAT ACCGTAAGCT TTAGCTCTTC TCCAAAAGTA CATAAGCCAT GCCTCCTTAA ACAGGACATC CTCAGATTCC TCTGGGCTAA GTTCAAATGC TTCCATATAC TTCGGATCAA TTGGTGATTT GAAGTCTGGC ATGGATGTCC TTGAAGAATT TGGACGTCTT TGTTGAGCAA ATGCCAGAAC CATAGATTCT TCAACCTTCA AGGAAGCTAG TTCTTTTAAG CCCATTTCAA CTGTTAGCAT GCTCTCAATA TTTCCGTCTC CAGTAAGATC ATTAAAGTCG TCAACAAGTT TACTCCTTTG CGGATGGTTT TCACCTTTGT TCCAGCACT |