Genetic Maps March 23, 2009 ----------- This directory contains the gentic maps created by the Mimulus project. The file list and brief description are as follows: IM62_x_NAS_F2N1_2006.txt -- Created by Lilla Fishman and all M. guttatus (IM62) x M. nasutus (SF) F2N1 2006 IM62_x_DUN_Rils_2009.txt -- Created by Willis Lab at Duke. From a cross between IM62 and an outbred plant from the Oregon Dune population - - a single F1 was selfed to produce F2's which were selfed 3-5 additional generations. Lewisii_x_Cardinalis_2009.txt -- From Toby Bradshaw M. lewisii x M. cardinalis (2009) IM62_x_NAS_F2_2009.txt -- From Wilis Lab at Duke M. guttatus (IM62) x M. nasutus (SF) F2N2 2009 Irn_Mtn_Combined_2009.txt -- From Willis Lab at Duke This map is a consensus map of three independent F2 intrapopulation crosses. Genetic materials were obtained in a selection experiment for corolla width (N=1000, selection intensity 20%, six generations, high and low). All individuals were outbred in the selection experiment. We crossed high/low trait value parents from the distribution extremes to generate 3 independent F1s, then selfed the F1s to get three F2 populations (N=384 each) All map construction was done in JOINMAP. Independent maps were generated for each cross with the ML mapping algorithm. Then marker orderings from each independent map, as well as marker orderings from scaffolds in the 6x genome, was used as fixed orders to create the integrated map. Markers which resulted in wrong orderings or negative orderings were deleted (this could be due to random variation between crosses in recombination frequency, misgenotyping, or transmission ratio distortion heterogeneity between crosses).